Biochemical Characterization of the Rice Kinesin O12 and N14 Belonging to Kinesin-14 Family
نویسندگان
چکیده
منابع مشابه
Microtubule organization by the antagonistic mitotic motors kinesin-5 and kinesin-14
During cell division, different molecular motors act synergistically to rearrange microtubules. Minus end-directed motors are thought to have a dual role: focusing microtubule ends to poles and establishing together with plus end-directed motors a balance of force between antiparallel microtubules in the spindle. We study here the competing action of Xenopus laevis kinesin-14 and -5 in vitro in...
متن کاملKar3Vik1, a member of the Kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern
Kinesin-14 motors generate microtubule minus-end-directed force used in mitosis and meiosis. These motors are dimeric and operate with a nonprocessive powerstroke mechanism, but the role of the second head in motility has been unclear. In Saccharomyces cerevisiae, the Kinesin-14 Kar3 forms a heterodimer with either Vik1 or Cik1. Vik1 contains a motor homology domain that retains microtubule bin...
متن کاملStructures of kinesin and kinesin-microtubule interactions.
Several X-ray crystal structures of kinesin motor domains have recently been solved at high resolution ( approximately 0.2-0.3 nm), in both their monomeric and dimeric states. They show the folding of the polypeptide chain and different arrangements of subunits in the dimer. In addition, cryo-electron microscopy and image reconstruction have revealed microtubules decorated with kinesin at inter...
متن کامل[Kinesin-14 leaps to pole position in bipolar spindle assembly].
Formation of a mitotic spindle occurs almost effortlessly as cells cycle and is essential for chromosome segregation. Early in a cell cycle, the replication of centrosomes or spindle pole bodies at G1/S leaves mother and daughter poles in close association until forced apart into a bipolar arrangement at mitotic onset. Kinesin-like proteins (Klps) generate a variety of forces that contribute to...
متن کاملCell Biology: Kinesin-14 Backsteps to Organize Polymerizing Microtubules
Microtubules nucleated from an organizing center grow radially in all directions. A new study shows that, to organize those microtubules into arrays of parallel bundles, the kinesin-14 Cik1-Kar3 guides growing microtubule tips along pre-existing microtubules.
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2012
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2011.11.2017